聚合物AQ-二甲基二茂铁修饰微组合电极的电化学行为及葡萄糖传感器的研究

ELECTROCHEMICAL BEHAVIOR OF EASTMAN AQ POLYMER/DMFe MODIFIED MICRODISKARRAY ELECTRODE AND ITS APPLICATION TO GLUCOSE ANALYSIS

本文用聚合物AQ(Eastman-AQ)将二甲基二茂铁、葡萄糖氧化酶固定在微组合电极上制成了葡萄糖微传感器。二甲基二茂铁作为一个介体将电子从电极传递给葡萄糖氧化酶(GOD)。催化电流与葡萄糖浓度在1～12mmol/L呈线性关系,探讨了温度、pH对催化电流的影响。微传感器的响应时间小于15秒,AQ膜的阳离子交换特性和很强的附着力以及二甲基二茂铁的工作电位低,使微传感器的稳定性有所提高,并且消除了抗坏血酸、尿酸的干扰。血清中葡萄糖浓度的测定与分光光度法的结果基本相符。

An enzyme microeleetrode for glucose determination has been prepared from glucose oxidase and dimerhylferrocene (DMFc) immobilized in Eastman-AQ film on microdisk array of carbon fibers.The ferrocene derivative couple(Fc/Fc^+) plays the role of mediator for electron transfer between immobilized glucose oxidase and the electrode. A linear current response to the glucose concentration over a range of 1～12mmol/L was observed at this en zyme microelectrode. The influences of pH and temperature on the enzyme electrode behavior are also presented. The response times was found to be less than 15s. The interference of the electroactive species such as ascorbic and uric acid could be effectively depressed. The resuits of analysis of serum samples were in good agreement with those obtained by photometric analysis.