以聚邻苯二胺修饰的镀铂玻碳电极为基底的葡萄糖传感器

A GLUCOSE SENSOR BASED ON POLY-1,2-DIAMINOBENZENE MODIFIED PLATINIZED GLASSY CARBON ELECTRODE

生物电化学传感器所面临的主要问题,就是干扰和污染。具有选择渗透性的聚合物膜可以防止电活性物质到达电极表面和大分子物质对基底电极的污染,提高电极的选择性,延长使用寿命。这方面的报道已很多。电聚合的邻苯二胺膜的选择渗透性已有报道。其优点是薄(<10nm),且具有自身的绝缘性,可以被均匀地聚合在电极表面。我们用这种聚合物膜修饰电极为基底电极,化学交联法将葡萄糖氧化酶(GOD)固定在基底电极表面。

A platinized glassy carbon electrode (GCE), electropolymerized with 1,2-diaminobenzene and immobilized with glucose oxidase(GODx), is used in the construction of a sensor for the determination of glucose. The platinum coating provides an increased current response to the oxidation of bydrogen peroxide as compared with a bare GCE. The permselectivity of 1,2-diaminobenzene polymer can drastically reduce the effects of electroactive interferents, such as aseorbie acid and uric acid, and prevent high molecular weight species from fouling on the electrode surface. The sensor remains the advantages of conventional GODx electrode such as high response, wide linear range, fast response and has high selectivity and reproducibilty.