间隔补铁大鼠的肠粘膜铁含量及其生物利用率

INTESTINAL MUCOSAL IRON LEVELS AND ITS UTILIZATION IN INTERMITTENTLY Fe SUPPLEMENTED RATS

雄性SD断奶大鼠32只,随机分为缺铁和铁正常两组。喂纯合成饲料,铁含量分别为0与0.8mg,每日两次喂饲。12天后再各分为每日补铁组与间隔三日补铁组,铁剂量每日与每间隔三日均为8mg,同前方式两次喂饲。于第一次补铁时喂入^(59)Fe 3.7×10~4Bq。18天后杀死大鼠,对十二指肠与小肠各段粘膜铁、肠腔遗留铁及其^(59)Fe(cpm)进行检测,并计算出^(59)Fe利用率。结果表明,每日补铁组肠粘膜与肠腔遗留铁分别为铁正常对照组的8与20倍。^(59)Fe利用率为其1/2。间隔补铁组肠粘膜铁接近铁正常对照组,^(59)Fe利用率为其2倍,肠腔内遗留铁仅为每日补铁组1/5。间隔外铁组补铁剂量小,副作用明显少于每日补铁组,肠粘膜铁吸收与利用比较理想。

Thirty two male weanling Sprague Dawley rats were made iron-deficient or normal-iron by feeding AIN-76 Specifications without or with 0.8mg Fe (divided twice daily). After 12 days both groups of rats were subdivided into two groups, each one received 8mg Fe at intervals of 3 days or daily respectively for 18 days, 3.7×104Bq of 69Fe were added to the first supplemented dose. Measurements included every segment intestinal mucosal Fe and the remaining Fe in washes in intestine, and thier cpm of 58Fe at sacrifice. 59Fe utilization were counted. The results showed that Fe content of intestinal mucosa and washes in daily Fe supplemented rats were from 8 to 20 times that of iron normal control group and 59Fe utilization was doubled. Intestinal mucosal Fe levels were close to that of iron-normal rats. Remaining Fc content in washes was 1/5 of daily Fe supplemented rats. So intermittent Fe supplementation was more efficient and the dose was small and side effect was less than daily supplementation and diminished intestinal Fe loading.