大鼠对富硒平菇硒的生物利用率

BIOAVAILABILITY OF Se IN Se ENRICHED MUSHROOM IN RATS

本实验用低硒大鼠对人工栽培富硒平菇作了硒生物利用率观察。大鼠按体重随机分为9组,其中1组为低硒对照组,2～5组补充Na_2SeO_3,饲料内硒含量分别为:0.51、0.75、1.91、3.18ppm;6～9组补充富硒平菇,硒为0.45、0.72、1.59、3.41ppm。3周后处死大鼠,采血、肝、测定硒含量,并测定红细胞GSH-Px酶活性。结果表明:血、肝硒值及红细胞GSH-Px酶活性均随补硒浓度的增加而增加,以亚硒酸钠为基准,富硒平菇硒生物利用率,按血硒指标为66.66％;按肝硒指标为125％;按红细胞GSH-Px酶活性指标为123.42％,说明人工栽培富硒平菇可作为极好的食物硒来源。

Bioavailability of selenium in Se enriched mushroom, Pleurstus ostreatus, was assayed with Se depleted rats. The selenium status in rats including blood, liver Se levels and erythrocyte glutathione peroxidase (GSH-Px) activity was evaluated. Young male SD rats were first fed with basal Se deficient diet for 4 weeks, followed by either continued depletion or repletion for another 3 weeks with 0.51, 0.75, 1.91, 3.18μg Se as sodium selenite, and 0.45, 0.72, 1.59, 3.41μg Se as Se enriched mushrooms per gram of diet for each group of rats respectively. Both Se levels in blood and in liver and erythrocyte GSH-Px activity were enhanced significantly with the increasing dietary Se contents. The relative bioavailability of selenium (taken selenite as 100%) in the Se enriched mushroom (P.ostreatus) was 66.66% based on the criteria of blood Se level, or 125.0% of liver Se level, or 123.42% of erythrocyte GSH-Px activity respectively. These results demonstrated that Se enriched mushroom (P.ostreatus) was an excellent dietary Se source.