结膜痕迹细胞学方法的研究与应用

ASSESSMENT OF VITAMIN A STATUS IN CHINA BY THE MODIFIED CONJUNCTIVAL IMPRESSION CYTOLOGY (CIC) METHOD

实验对结膜痕迹细胞学方法(CIC)进行了研究并加以改进。用0.45μ醋酸纤维膜对不同人群进行采样,以FAA液固定,固定时间可达两月之久,用过碘酸—Schiff试剂将球状细胞和粘蛋白染成粉红色,用Delafield苏木精将上皮细胞染成紫红色,即可进行镜检。实验采用48孔染色架可大大提高染色效率。包括婴幼儿在内的采样成功率可达97％以上。根据上皮细胞,球状细胞和粘蛋白的大小,形态将维生素A营养水平分为正常、临界、缺乏三个水平及相应的六个阶段。实验对不同地区不同年龄组的904人进行采样分析发现:农村儿童维生素A缺乏率(18.8％)大大高于城市儿童(0),农村青少年11.4％的缺乏率高于城市青少年(3.0％),成年人基本正常。对CIC方法与血清维生素A浓度法进行比较,结果两者呈正相关(r=0.78)

Conjunctival Impression Cytology (CIC) method was modified to assess vitamin A level more exactly and simply in China. The infants (<2 years) were sampled during their deep sleep, and the successful rate was 93%. Delafield Hematoxylin instead of Harris Hexatoxylin was used to make the staining well. Eight hanbred and ninety-six subjects different ages in different areas were surveyed. There was no vitamin A deficiency sign in urban (Beijing) children, but about 18.8% of rural (Laiyuan, Hebei) children were detected to have low serum vitamin A level. The CIC level in most of the children with the mucous membrane disease was low. The teenager's vitamin A deficiency in rural (Gaocheng, Hebei) (11.4%) was more than that in the urban (Beijing) (3.0%). The vitamin A nutrition was normal in adults. As the CIC method was well correlated to the serum vitamin A concentration method, could be used to assess vitamin A status.According to the nutrition survey in China in 1982, 0.9% people were found to have vitamin A deficiency, but there were more people with poor vitamin A status if CIC method was used,so the situation should further be investigated.