醒脑启智胶囊药物血清对PC12细胞缺糖损伤的保护作用

Protective effect of xingnao qizhi capsule drug serum on PC12 with sugar defici ency injury

目的:探讨缺糖对PC12细胞的影响以及醒脑启智胶囊药物血清对其的保护作用。方法:采用血清药理学方法,体外培养PC12细胞,用无糖Earle's液产生缺糖损伤,观察PC12细胞形态学、MTT法细胞活力、乳酸脱氢酶(lactatedehydrogenase,LDH)活性的变化。结果:PC12细胞在缺糖作用下有明显的损伤,镜下可见细胞肿胀圆缩,折光性下降,贴壁功能下降,部分细胞裂解成碎片;给予醒脑启智胶囊药物血清均能明显减轻缺糖对PC12细胞形态学变化的影响,表现为细胞折光性较好,细胞碎片形成减少。缺糖损伤后的PC12细胞活力降低0.20±0.03,LDH活性升高犤(1.85±0.23)kU/L,加入醒脑启智胶囊犦血清后,细胞活力明显增高,LDH活性明显降低。结论:醒脑启智胶囊药物血清能明显减轻PC12细胞的缺糖损伤,并呈现一定的剂量依赖关系。

AIM:To investigate the effect of sugar deficiency on cell PC12 and the protect ive function of xingnao qizhi capsule drug serum. METHODS:Cell PC12 was cultured in vitro with the method of serum pharmacology. Moreover,the injury of sugar deficiency was induced by Earle's solution without sugar,so as to observe the morphology of PC12,cell vitality with MTT and the cha nges of lactate dehydrogenase(LDH) vitality. RESULTS:PC12 was injured obviously under the sugar deficiency and could be obs erve with cell turgescent and ring diminution,descent of reflected light and anc horing function, and part cell splitting into fragment.Xingnao qizhi capsule dru g serum could relieve the effect of sugar deficiency on the changes of morpholog y of PC12.The results showed that the reflected light was strengthened and the n umber of fragment was decreased.After sugar deficiency injury,the vitality of P C12(0.20±0.03) was decreased,but the vitality of LDH[(1.85±0.23) kU/L]was in cr eased. However,the vitality of cell was obviously increased and the vitality of LDH was markedly decreased after given xingnao qizhi capsule drug serum. CONCLUSION:Xingnao qizhi capsule drug serum can obviously alleviate the sugar deficiency injury of PC12,which presents a certain relation on the dosage.