妥曲珠利在鸡组织内的残留研究

Study on residue of toltrazuril in chicken's tissue

研究鸡单剂量喂服 (5 0mg/kg·d ,10 0mg/kg·d ,连服 2d)妥曲珠利后在肝脏、肾脏、肌肉组织中的残留量 ,以便制定妥曲珠利预混剂的休药期。用乙腈 -乙酸乙酯 (3/ 2 ,v/v)提取组织中的药物 ,利用高效液相色谱法测定组织中妥曲珠利的浓度。试验结果表明 ,10 0mg/kg饲料添加妥曲珠利在鸡体内休药期为 14d ,5 0mg/kg时休药期为 8d。该结果与农业部制定的无公害食品饲养兽药使用准则中所规定的 75～ 10

According to the tests for antiparasite activity and clinical efficiency of toltrazuril produced by XinYa pharmaceutical corporation, ShangHai, the optimal additive dosage to chicken fodder is confirmed as 25～50 mg/kg. In order to establish the withdrawal time, residue of Toltrazuril in liver, kidney and muscle was investigated following single oral administration to chicken (50 mg/kg,100 mg/kg, once daily, amount to 2 days)in this experiment. The drug was extracted by acetonitrile-acetate acid(3/2,v/v) and determined using HPLC. The chromatographic condition: Kromasil-C18 column(250×4.6 mm,5 um), UV detector with wavelength fixed at 254 nm, the mobile phase selected as actonitrice-pBs(60/40), flow rate set at 1.0 ml·min^(-1). The limit of detection(Lob)is long; the lowest detectable concentration is 10 μg/kg. The results showed that the withdrawal time for 100 mg/kg dose is 14 d and that for 50 mg/kg is 8 d, which is consistent with the regulations of safe food and breed veterinary medicine constituted by CAA(China Agriculture Administration) with the withdrawal time of Toltrazuril for 75～100 mg/kg dose.