红菜薹游离小孢子培养与植株再生

Isolated Microspore Culture and Plant Regeneration in Purple Flowering Stalk (Brassica compestris ssp. chinensis var. pupurea Hort.)

以5个红菜薹(Brassicacompestrisssp.chinensisvar.pupureaHort.)基因型为试材,探讨了基因型和活性炭对产胚量的影响。结果表明:产胚量最高的是基因型8902,达到42个/皿,最少的为零;加适量活性炭可以使产胚量提高近3倍。同时,对胚状体进一步再生成苗因素也进行了研究:在培养基中添加1.2%的琼脂浓度再生率最高,达到50.1%;4℃下处理10d可使再生成苗率从45%提高到65%;随胚状体年龄的延长,其再生成株率明显降低,最适的胚龄是20～24d;而培养基B5和MS对小孢子再生率的影响不大。

The influences of the genotype and the activated charcoal were investigated on the embyroid yields of microspore in purple flowering stalk(Brassica compestris ssp. chinensis var.pupurea Hort.)among 5 genotypes. The results showed, the highest mean yield was up to 42 embryoids per petri dish, while the lowest was zero. The efficiency of embryoid increased by 3-fold when the activated charcoal was added into the liquid media properly. The embryoids produced from microspore culture were further conducted to regenerate plantlet. The regeneration frequency was the highest (up to 50.1%) while solidified with 1.2% of agar in the medium. When treated at 4℃ for 10 days, the regeneration frequencies of the embryoids rose from 45% to 65%.The optimal ages of embryoids (20-24 d) were vital to plant regeneration, the regeneration frequency decreased remarkably along with the increase of the age of embryoids. The media, B5 and MS, had no distinct difference for plant regeneration.