大鼠输卵管沙眼衣原体感染后体内树突状细胞的变化

VARIATIONS OF DENDRITIC CELLS IN VIVO FOLLOWING CHLAMYDIALINFECTION OF OVIDUCTS

目的观察大鼠输卵管沙眼衣原体(CT)感染后体内树突状细胞(DC)的变化。方法选择成年雌性Wistar大鼠,从卵巢囊接种沙眼衣原体E型株。于感染后第1/2d、7d、14d、21d、28d和第35d处死动物取材,显示输卵管和脾内DC的变化。结果1.实验1/2d组输卵管未出现S100+DC,在实验第7d、14d、21d、28d和第35d组输卵管均有S100+DC出现;2.感染后脾淋巴小结数量增多,体积较大,淋巴小结内S100+DC密集,动脉周围淋巴鞘内S100+DC增多。结论输卵管CT感染后,输卵管和脾内S100+DC增加,提示在体内DC对CT具有抗原提呈和启动机体免疫应答的作用,DC具有作为CT治疗性疫苗基础构建的潜力。

? Objective To study the variations of dendritic cells (DC) in vivo after the chlamydial infections of oviduct. Methods Female Wistar rats were inoculated with chlamydial trachomatis (CT), immunotype E, into the ovarian bursa. The rats were killed on the 1/2 day, 7thday, 14thday, 21stday, 28thday and 35thday after the infection, respectively. The DC of oviducts and spleen were detected by immunohistochemistry. Results 1. DC did not appear in the oviduct of the 1/2 day test group but appeared in the 7th day, 14th day, 21st day, 28th day and 35th day test groups. 2. Lymphoid nodules in the spleen increased both in number and volume in the test groups than in the controls. There were abundant DC in splenic lymphoid nodules. The DC in the periarterial lymphatic sheath also increased. Conclusion After CT infection, the DC in vivo can pressnt the antigen and activate the immune response, which indicates that DC may be used as a vaccine against CT.〔