人乳腺癌中3种耐药基因产物的表达及其与预后的相关性

Protein Expression and Correlation with Prognosis of Three Drug Resistant Genes in Human Breast Carcinoma

目的 研究多药耐药基因产物P-糖蛋白(P-gp)、谷胱甘肽-S转移酶π(GST-π)和DNA拓扑异构酶Ⅱα (TopoⅡα)在乳腺癌组织中的表达及其与预后指标的关系。方法 采用免疫组织化学技术,检测105例乳腺癌 中P-gp、GST-π和TopoⅡα耐药基因蛋白。结果(1)105例乳腺癌中P-gp、GST-π和TopoⅡα的阳性表达率分 别为77.1％、76.2％和36.2％;(2)P-gp及GST-π高表达和TopoⅡ α的低表达与肿瘤的浸润性和腋窝淋巴结转 移相关;(3)P-gp、GST-π和TopoⅡα与C-erbB-2的表达显著正相关;(4)GST-π与ER、PR的表达呈负相关。结 论P-gp、GST-π和TopoⅡα在乳腺癌的表达与其预后指标存在一定的联系,对乳腺癌患者在化疗前进行耐药 基因检测,有助于判断预后及选择合理的化疗方案,从而提高疗效。

Purpose To investigate the protein expression and the correlation with prognostic factors of drug resistant genes P-glycoprotein (P-gp) , glutathoine S-transferase Pi (GST-π) and topoisomerase Ⅱ α(Topo Ⅱα) in breast carcinoma. Methods P-gp, GST-π and Topo Ⅱα protein expression in 105 cases of breast carcinoma were detemined using immunohistochemistry assay. Results (1) Positive expession rates of P-gp, GST-π and Topo Ⅱα in 105 cases of breast carcinoma were 77.1 % ,76.2 % and 36.2 % , respectively; (2) There was a significant relationship between the higer expression of P-gp, GST-π, lower expression of TopoⅡαand the tumor invasion and the axillary lymph node involvement; (3)P-gp,GST-πand TopoⅡα siginificantly positively correlated with C-erbB-2; (4) GST-π significantly negatively correlated with ER and PR. Conclusions The expression of P-gp,GST-π and TopoⅡα in breast carcinoma correlated with the prognostic factors. Before chemotherapy to patients with breast carcinoma, the test of drug resistant genes should be done, which will help doctors predict prognosis and select a reasonable chemotherapy regimen so as to improve curative effect.