产木聚糖酶的沿海红树林真菌筛选及其培养与酶活测定条件优化(英文)

Survey of coastal mangrove fungi for xylanase production and optimized culture and assay conditions

从香港海岸红树林分离到的 77株真菌中有 34株可产生木聚糖酶 ,从中选出CY2 80 9(Staganosporasp .)、CY4 786和CY5 0 4 0等 3菌株与已知陆生产酶菌株HU5 0 4 8(Aspergillusawamori)进行产木聚糖酶的比较研究。根据培养液中菌丝生物量、木聚糖酶活力和木糖等价还原糖含量等指标的测定 ,菌株CY4 786在起始pH 7 8的木聚糖 酵母膏 海盐液体培养基中 2 5℃下震荡 (10 0r min)培养 7d产酶最佳 ;粗酶液在 5 0℃和pH 4 6的优化条件下进行测定 ,木聚糖酶活力达到 1 0 7× 10 4 U mL。结果表明 ,红树林真菌起着半纤维素降解者的作用 ,沿海红树林环境中存在着可资利用的木聚糖酶产生菌。

Xylanase activity was detected among 34 of 77 fungal isolates derived from decaying wood, debris and soil samples collected in coastal mangrove environment of Hong Kong. Of those, three isolates CY2809 (Staganospora sp.), CY4786 and CY5040 were chosen for comparison of xylanase production in parallel to HU5048 (Aspergillus awamori), a terrestrial, highly productive isolate. Based on the assessment of mycelial biomass, xylanase activity and content of xylose-equivalent reducing sugars in their liquid cultures, the isolate CY4786 was best for xylanase production in a basal medium containing birchwood xylan (10.0 g/L) as a sole carbon source, yeast extract (2.5 g/L) and sea salts (15.0 g/L) with initial pH 7.8. When assayed at the optimized regime of 50℃ and pH 4.6, the activity of xylanase produced by CY4786 in 7d liquid culture at 25℃ reached 1.07×10 4 unit/mL. The results indicate that the mangrove fungi act as hemicellulose decomposers in the mangrove environment where highly xylanase-productive isolates can be searched for exploitation. A discussion is given on the possible use of the content of xylose-equivalent reducing sugars as an index to simplify conventional xylanase activity assay method for fungal isolate survey.