电针足三里对酸化乙醇致大鼠胃黏膜损伤胃组织总SOD、MDA影响的时效关系研究

Time-efficacy Relationship in the Influence of Electroacupuncturing Zusanli on Gastric Tissue Total SOD and MAD in Acidified Ethanol-induced Gastric Membrane Injury in Rats

目的 通过电针足三里对酸化乙醇致大鼠胃黏膜损伤模型胃黏膜影响的实验 ,探讨针刺的时效关系。方法 将动物随机分组、造模 ,造模形成后电针 ,在电针后 7个时刻分别取材、检测胃组织总SOD与胃组织MDA。结果 针刺后 3h、12h时段SOD活性均值与相应模型组均值比较P <0 .0 5有显著差异 ,针刺组与对应时间模型组动物指标均值曲线有一定变化趋势 ,其中针刺 3组比其对应模型 3组胃组织总SOD相对明显升高 ,胃组织MDA相对明显降低。结论 本实验各针刺组治疗 1次后 ,不同的时段对胃组织总SOD与胃组织MDA的影响不同。结果显示针刺对提高胃组织总SOD的活性、降低胃组织MDA的含量有一定的作用 ,尤其在针刺后 3h时段其修复作用较明显。

Objective To investigate acupuncture time-efficacy relationship by the influence of electroacupuncturing Zusanli on gastric membrane in acidified ethanol-induced gastric membrane injury in rats.Method The animals were randomly divided into groups. Electroacupuncture was performed after the model was made. The gastric tissue was taken at 7 points of time after electroacupuncture and its total SOD and MAD were measured.Results The average value of SOD activity at 3 and 12 hours after acupuncture was significantly different from that in the corresponding model groups (P<0.05); the curve of average index value had a certain changing tendency in both acupuncture and corresponding model groups, of which, gastric tissue total SOD was relatively significantly higher and gastric tissue MAD relatively significantly lower in acupuncture group 3 than in model group 3.Conclusion One session of treatment has different effects on gastric tissue total SOD and gastric tissue MAD in different periods of time. Acupuncture has a certain increasing effect on gastric tissue total SOD activity and a certain lowering effect on gastric tissue MAD content, which is especially marked at 3 hours after acupuncture.