聚焦超声对肝癌细胞SMMC7721增殖能力的影响

Effects of focused ultrasound on capability of proliferation of human hepatocarcinoma cell SMMC7721 in vitro

目的研究聚焦超声对肝癌细胞SMMC7721增殖能力的影响,初步探讨产生该影响的机制。方法以剂量为0.8MHz20W5s的聚焦超声(该剂量照射过的SMMC7721细胞可保持70%的存活率)照射处于对数生长期的SMMC7721细胞,用免疫组化法及原位杂交法观察SMMC7721生化及基因表达的变化。结果聚焦超声抑制SMMC7721细胞VEGF和PCNA蛋白的表达(P<0.05)以及VEGF和PCNAmRAN的表达(P<0.05)。结论用聚焦超声照射后存活的SMMC7721细胞增殖能力减弱,其机制可能是通过抑制VEGF和PCNAmRNA的表达,使VEGF和PCNA蛋白表达减少来实现。

Objective:To reseach the effects of focused ultrasound on the capability of proliferation that human hepatocarcinoma cell SMMC7721 have in vitro,and to determine the mechamism of this therapy initially.Methods:To irradiate the SMMC7721 cell which is in logarithmic growth time with ultrasound.The dosage of ultrasound was 0.8MHz 20W 25s,which make 70% of SMMC7721 cell that had been irradiated alive.The changes in cell morphology,biochemistry and gene expression were observed with immunohistochemistry and hybridization in situ.Results:The ultrastructure of SMMC7721 cell was changed by focused ultrasound,and the ultrasound could inhibit the expression of VEGF,PCNA,VEGF mRNA and PCNA mRNA( P <0.05).Conclusion:The capability of proliferation of human hepatocarcinoma cell SMMC7721 was weakened by irradiation of focused ultrasound.The mechanism perhaps is that the irradiation of focused ultrasound inhibits the expression of VEGF mRNA and PCNA mRNA.