氧化苦参碱对放疗鼻咽癌HNE-1细胞P-糖蛋白表达影响

Effects of oxymartine on expression of P-glycoprotein in HNE-1 cell line radiation therapy

目的(1)氧化苦参碱对放射前后鼻咽癌HNE-1细胞有无生长抑制作用;(2)研究天然产物氧化苦参碱对鼻咽癌HNE-1细胞株放射前、后P-糖蛋白表达的影响。方法(1)MTT法检测长春新碱、维拉帕米、氧化苦参碱对放射前HNE-1细胞、放射后HNE-1(200)细胞生长抑制情况;(2)免疫化学法检测氧化苦参碱、维拉帕米对HNE-1(200)细胞P-糖蛋白表达的影响。结果(1)长春新碱、维拉帕米、氧化苦参碱对放射前、后细胞生长均有抑制作用,呈浓度依赖关系,随着浓度的增加抑制作用也增加;(2)高浓度VCR对放射治疗后细胞HNE-1(200)的抑制率比对HNE-1细胞的抑制率降低了近50%,P值小于0.01,有显著差异;氧化苦参碱、维拉帕米对两种实验细胞抑制没有显著差异。(3)免疫组化检测细胞P-糖蛋白表达,结果为HNE-1阴性,HNE-1(200)阳性,维拉帕米、氧化苦参碱分别作用于HNE-1(200)细胞后P-gp表达弱阳性。结论(1)氧化苦参碱对放射前后鼻咽癌HNE-1细胞有明显生长抑制作用;(2)放射后细胞HNE-1(200)对长春新碱产生耐药;(3)低毒性浓度氧化苦参碱具有与维拉帕米相似的作用,能使放射照射后鼻咽癌HNE-1细胞株P-糖蛋白的表达下降。

Objective:To investigate the effects of oxymartine on cell growth and the changes of P-glycoprotein in HNE-1 cell line after exposed to fractionated X-irradiation.Methods:By MTT,the cell proliferation was observed both before X-irradiation and after X-irradiation conditions with the extent of vincristine,oxymartine and verapamil respectively.P-glycoprotein expression was tested by immunohistochemistory method SP.Results:(1)Vincristine,oxymartine and verapamil were capable of inhibiting cell growth on HNE-1 cell line and fractionated X-irradiated HNE-1(200)cell subline;(2)Vincristine was less inhibitory on cell growth in HNE-1(200)cell subline than HNE-1 cell line,and the difference had statistic significance;(3)P-glycoprotein expression in HNE-1 cell line was negative while HNE-1(200)cell subline positive;oxymartine with low inhibitory concentration could increase the expression of P-glycoprotein in HNE-1(200) cell subline,which is similar to that of verapamil.Conclusion:(1)Oxymartine is capable of inhibiting cell growth on HNE-1 cell line and fractionated X-irradiated HNE-1(200)cell subline;(2)HNE-I(200)cell subline is resistant to vincristine;(3)Oxymartine with low inhibitory concentration could increase the expression of P-glycoprotein in HNE-1(200)cell subline.which is similar to that of verapamil.