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人脂联素cDNA的克隆及序列分析 被引量：5

Cloning and Sequencing of cDNA of Human Adiponectin
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摘要 目的:构建人脂联素cDNA的克隆并进行序列分析。方法:提取人脂肪组织总RNA,运用RT-PCR方法扩增人脂联素cDNA完全编码区,将扩增产物通过TA克隆技术克隆至pMD18-T载体,对重组质粒进行测序验证。结果:成功构建人脂联素cDNA的克隆,重组质粒(pMD18-T/ADPN)经测序与Genebank检索的脂联素cDNA序列进行对比证实为100%同源。结论:所构建的人脂联素cDNA克隆适于进一步的克隆表达。 Objective: To clone cDNA of human adiponectin for nucleotide sequencing. Methods: The total RNA of human adipose tissue was extracted.The cDNA complete coding region of human adiponectin was amplified by RT-PCR.The product was cloned into plasmid pMD18-Tby TA-clone.The recombinant plasmid was verified by sequencing. Results: The clone of cDNA of human adiponectin was successfully constructed.The recombinant plasmid(pMD18-T/ADPN)was verified to share 100%homologies with that of genebank by sequencing. Conclusion: The cloning of human adiponectin cDNA is adapted to further expression.
作者 刘德敏 靳立忠 孙颖 张捷
机构地区 天津医科大学代谢病医院
出处 《天津医药》 CAS 北大核心 2005年第2期71-73,共3页 Tianjin Medical Journal
基金 天津市教委资金资助项目(项目编号:20030325)
关键词 脂联素 CDNA序列 克隆表达 重组质粒 序列分析 测序 脂肪组织 T载体 克隆技术 CDNA克隆 proteins DNA, complementary cloning, molecular sequence analysis fats adiponectin
分类号 R587.1 [医药卫生—内分泌] R589 [医药卫生—内分泌]
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参考文献13

  • 1Weyer C, Funahashi T, Tanaka S, et al. Hypoadiponectinemia in obesity and type 2 diabetes: close association with insulin resistance and hyperinsulinemia. J Clin Endocrinol M etab, 2001, 86:1930-1935.
  • 2Yokota T, Oritani K, Takahashi I, et al. Adiponectin, a new member of the family of soluble defense collagens, negatively regulates the growth of myelomonocytie progenitors and the function of maerophages.Blood, 2000, 96:1723-1732.
  • 3Takahashi M, Arita Y, Yamagata K, et al. Genomic structure and mutations in adipose-specific gene, adiponeetin, Int J Obes, 2000, 24: 861-868.
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  • 5Arita Y, Kihara S, Ouchi N, et al. Parodoxical decrease of an adipose-specific protein, adiponectin, in obesity. Biochem Biophys Res Commun, 1999, 257:79-83.
  • 6Weyer C, Funahashi T, Tanaka S, et al. Hypoadiponectinemia in obesity and type 2 diabetes: close association with insulin resistance and hypefinsulinemia. J Clin Endocfinol Metab, 2001, 86:1930-1935.
  • 7Fruebis J, Tsao TS, Javorschi S, et al. Proteolytic cleavage product of 30-kDa adipocyte complement-related protein increases fatty acid oxidation in muscle and causes weight loss in mice.Proc Natl Acad sci USA, 2001, 98:2005-2010.
  • 8Berg AH, Combs TP, Du X, et al. The adipocyte-secreted protein Acrp30 enhances hepatic insulin action. Nat Med, 2001, 7:947-953.
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同被引文献27

  • 1杨泽华,解军,于保锋,杨琦,张悦红,牛勃.人脂肪细胞总RNA的抽提及对脂联素mRNA的扩增[J].中国药物与临床,2005,5(4):265-268. 被引量：4
  • 2刘德敏,杜春红,孙颖,张捷.绿色荧光蛋白标记的人脂联素真核表达载体的构建[J].天津医药,2007,35(5):324-325. 被引量：1
  • 3李丙蓉,郑丹,邓华聪,刘金波,兰丽珍,郑宏庭.人脂联素、球状脂联蛋白基因克隆及真核表达载体的构建与表达[J].中国糖尿病杂志,2007,15(6):367-369. 被引量：1
  • 4Arita Y, Kihara S, Ouchi N, et al. Paradoxical decrease of an adipose-specific protein,adiponectin, in obesity [J]. Biochem Biophys Res Commun, 1999, 257 (1): 79-83.
  • 5Manigrasso MR, Ferroni P, Santilli F, et al.Association between circulating adiponectin and interleukin-10 levels in android obesity: effects of weight loss[J]. Clin Endocrinol Metab,2005,90(10):5876- 5879.
  • 6Fruebis J, Tsao T, Javorsehi S, et al. Proteolycic cleavage product of 30-kDa adipocyte complement-related increases fatty acid oxidation in muscle and causes weight loss in mice[J]. Proc Natl Acad Sci USA, 2001,98(4) : 2005-2010.
  • 7Kadowaki T, Yamauchi T, Kubota N, et al. Adiponectin and adiponectin receptors in insulin resistance, diabetes, and the metabolic syndrome[J]. J Clin Invest, 2006, 116(7): 1784-1792.
  • 8Wang Y, Lam KS, Xu JY, et al. Adiponectin inhibits cell proliferation-dependent manner [J]. J Biol Chem, 2005, 280 (18): 18341- 18347.
  • 9Hotta K, Funahashi T, Arita Y, et al. Plasma concentrations of a novel, adipose-specific protein, adiponectin, in type 2 diabetic patients[J]. Arterioscler Thromb Vasc Biol, 2000,20(6):1595-1599
  • 10Weyer C, Funahashi T, Tanaka S, et al. Hypoadiponectin in obesity and type 2 diabetes: close association with insulin resistance and hyperinsulinemia[J]. Nat Med, 2001, 7(8): 941-946.

引证文献5

二级引证文献7

天津医药
2005年 第2期

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