嗅鞘细胞培养上清胶质源性神经生长因子含量的测定

Detection of glial cell line-derived neurotrophic factor in olfactory ensheathing cells supernatant

目的 观察嗅鞘细胞培养上清胶质源性神经生长因子 (GDNF)的含量随培养时间的变化关系 ,为选择嗅鞘细胞移植的最佳时间提供理论依据。方法 采用差速贴壁纯化培养方法培养嗅球及嗅粘膜嗅鞘细胞 ,培养 2 1天 ,3天留取标本一次 ,采用ELISA法对上清GDNF进行测定。结果 GDNF的含量随培养时间的延长逐渐升高 ,但绝对含量很低。结论 培养的嗅鞘细胞能分泌GDNF ,细胞移植在

Objective: To study the relationship that concentration of glial cell line-derived neurotrophic factor (GDNF) in olfactory ensheathing cells(OECs) supernatant was changed with time. Then provide the theoretic support for optimal time to transplant OECs. Method OECs were harvested from olfactory bulbs and olfactory mucosa, and cultured, purified by the method of the different rates of attachment among the various cells types. The OECs were cultured for 21days and harvested the samples every 3 days. The GDNF in the OECs supernatant was detected by the method of ELISA. Results The content of GDNF in OECs supernatant of olfactory bulb and olfactory mucosa raised gradually with cultured time, but the neat content was very low. Conclusion The cultured OECs can secret GDNF and the best time to transplant is at 14 days after cell culture.