胃肠富集kruppel因子GKLF在宫颈鳞癌中的表达及意义

Expression of GKLF/KLF4 in Squamous Crevical Carcinoma and its significance

目的 探讨胃肠富集kruppel因子 (GKLF)在宫颈鳞癌组织及正常宫颈组织中的表达及意义。方法 应用半定量的逆转录聚合酶链式反应 (RT -PCR)方法检测 32例宫颈鳞癌组织 (研究组 )中GKLFmRNA的表达强度 ,以 10例正常宫颈组织作为对照 (对照组 )。结果 GKLFmRNA在正常宫颈组织中的表达强度为 0 .76± 0 .15 ,而在宫颈鳞癌中的相对表达强度为 0 .4 2± 0 .19,与正常宫颈组织相比较 ,宫颈鳞癌组织中GKLFmRNA的表达丰度较低 (P <0 .0 5 )。而且临床分期愈晚 ,GKLFmRNA的表达强度愈低 ,差异有显著性 (P <0 .0 5 )。随着病理分级增高 ,GKLFmRNA的表达强度逐渐降低 ,差异有显著性 (P <0 .0 5 )。结论 宫颈鳞癌组织中GKLF表达下调 ,而且GKLFmRNA的表达与宫颈癌的临床分期和病理分级呈负相关 。

Objective: To investigate the expression of Gut-enriched kruppel like factor (GKLF/KLF4) mRNA and its significance in squamous cervical carcinoma and normal cervical epithelial tissues. Methods we compared GKLF expression in 32 cases of squamous cervical carcinoma to 10 cases of normal cervical epithelial tissues,utilizing semi-quantitative Reverse transcript-polymerase chain reaction (RT-PCR). Results The expression of GKLF mRNA in endometrial carcinoma is 0.42±0.19, while that is 0.76±0.15 in normal endometrium. Decreased expression of GKLF mRNA was observed in endometrial carcinoma patients compared to normal endometrial tissues, the difference is obviously(P<0.05).Furthermore, the expression of GKLF mRNA decreased with the clinical stage from 0 to Ⅳ, the difference was obviously(P<0.05). With the increasing of the pathologic grade from 1 to 3, decreased expression of GKLF mRNA was observed in endometrial carcinoma patients, the difference was obviously(P<0.05). Conclusion Decreased expression of GKLF in quamous cervical carcinoma and oppositely related to the pathologic grade and the clinical stage. suggest that GKLF may contributed to the development of cervical carcinoma.