摘要
Objective:To amplify antigen genes from patients with human immunodeficiency virus type 1 (HIV-1) in Guangdong Province for candidate AIDS vaccine design. Methods:Viral nucleic acid was isolated from 10 HIV-1 infected individuals' peripheral blood collected during 1995-2000 in Guangdong Province. The viral gag p24 gene and env gp120 gene were amplified by nested-PCR and sequenced. The homologies among HIV-1 isolates were compared with HIV-BLAST. Results: Among 10 HIV-1 isolates, nine are homologous to viruses of subtype B, and one is homologous to viruses of subtype E. Conclusion: Subtype B viruses of HIV-1 are predominantly present in Guangdong Province.
Objective: To amplify antigen genes from patients withhuman immunodeficiency virus type 1(HIV-1) in GuangdongProvince for candidate AIDS vaccine design. Methods: Viral nucleic acid was isolated from 10 HIV-1infected individuals' peripheral blood collected during1995-2000 in Guangdong Province. The viral gag p24 geneand env gp120 gene were amplified by nested-PCR andsequenced. The homologies among HIV-1 isolates werecompared with HIV-BLAST Results: Among 10 HIV-1 isolates, nine are homologous toviruses of subtype B, and one is homologous to viruses ofsubtype E. Conclusion: Subtype B viruses of HIV-1 are predominantlypresent in Guangdong Province.
基金
Financially supported by Natural Science Foundation of China(No 39870725)
Natural Science Foundation of Guangdong(No 980642)
Research Foundation of Guangdong Education Beaureu(No.20032).
