摘要
Objective:To establish a rapid and simple assay for the diagnosis of Chlamydia trachomatis (CT) infection. Methods BALB/c mice were immunized with SDS-PAGE purified major outer membrane protein (MOMP) from CT and the monoclonal antibodies were obtained subsequently. Two-site ELISA was developed to detect CT infection. Results: The established assay was able to detect as low as 1.248ug/ml MOMP with interrun and inrun CV 6.9% and 3.1% respectively. 94% (34/36) of culture-positive samples were found to be positive in the current examination, indicating the high sensitivity of this assay. Conclusion: The assay is applicable for clinical diagnosis of CT infection.
Objective: To establish a rapid and simple assay for thediagnosis of Chlamydia trachomatis(CT) infection. Methods BALB/c mice were immunized with SDS-PAGEpurified major outer membrane protein (MOMP) from CTand the monoclonal antibodies were obtained subsequently.Two-site ELISA was developed to detect CT infection.Results: The established assay was able to detect as low as1.248ug/ml MOMP with interrun and inrun CV 6.9% and3.1% respectively. 94% (34/36) of culture-positive sampleswere found to be positive in the current examination,indicating the high sensitivity of this assay. Conclusion: The assay is applicable for clinical diagnosis ofCT infection.
