旱獭肝组织中嗜肝病毒表面抗原表达的免疫组化研究

Immunohistochemical study of hepadnavirus surface antigen expression in liver tissue of marmota baibacina.

目的 从组织学和免疫学角度寻找旱獭嗜肝病毒感染的证据。方法 应用免疫组织化学技术以土拨鼠肝炎病毒表面抗原抗体检测101份旱獭肝组织中嗜肝病毒表面抗原的表达,同时观察旱獭肝组织常规病理改变,并对抗原检出与病理组织改变的关系进行相关分析。结果 旱獭肝组织中嗜肝病毒表面抗原检出率为82.2％(83/101)。阳性抗原颗粒定位于肝细胞胞浆和/或胞膜,阳性细胞呈散在、簇状和片状分布。101份肝组织标本中14份出现肝炎的病理改变,且与抗原检出之间存在明显的相关性(r=0.92)。结论 首次应用免疫组织化学技术证实早獭存在类似土拨鼠肝炎病毒的嗜肝病毒感染,此种动物有可能用于建立嗜肝病毒感染动物模型。

Objective To investigate the evidence of hepadnavirus infection of grey marmota (Marmota baibacina). Methods Hepadnavirus surface antigen in liver tissue of grey marmots was detected by using immunohistochemical staining with antibody against woodchuck hepatitis virus surface antigen (Anti-WHs). Histopathologic activity and its correlation to the expression of the surface antigen were also studied. Results Hapadnavirus surface antigen was detected in liver tissue of 83 out of 101 animals (82.2%). The stained substance located in the cytoplasm and/or membrane of liver cells, and the stained cells distributed scatterly, clusterly or diffusely. Mild inflammation was evident in 14 liver sections and it corrdated to hepadnavirus surface antigen expression. Conclusion By using immunohistochemical method, it was firstly proved that hepadnavirus similar to woodchuck hepatitis virus infection existed in grey marmota. Grey marmota may be used as an animal model to study hepadnavirus infection.