大白菜游离小孢子培养

Isolated Microspore Culture of Chinese Cabbage (Brassica campestris ssp. pekinensis)

对13个大白菜品种(品系)进行了游离小孢子培养,从12个品种中诱导出胚,最高诱导效率为每花蕾平均产胚178个。对培养小孢子的发育过程进行了系统观察,结果表明,接种后小孢子先于35℃高温下处理一天,对小孢子发育成胚十分必要。本结果为芸薹属作物花药培养和游离小孢子培养提供了细胞形态学方面的依据。

Isolated microspores from 13 varieties of Chinese cabbage (Brassica campestris ssp. pekinensis) were cultured, the results showed that yields of microspore-derived embryos were different among varieties. The highest frequency obtained was 178 embryos per bud. Developmental events of microspores cultured under different temperature treatments were systematically examined. The results demonstrated that microspores incubated at 35℃ for one day prior to culture at 25℃ was essential to high yield of microspore-derived embryos. The cytological events of cultured microspores showed that elevated temperature could prevent cultured microspores from developing in the direc- tion of mature pollens, which was beneficial to the induction of microspore-derived embryos.