ABA/GAs调控特异蛋白质与柑桔的抗寒性

The Relationship between Specific Proteins Regulated by ABA / GAs and Cold Resistance of Citrus Plants

选用较抗寒柑桔品种兴津温州蜜柑(Citrus unshiu Marc.)枳砧盆栽苗为材料,研究了人工低温锻炼和外源 ABA喷布处理下其内源 ABA/GAs对抗寒特异蛋白质的调控作用及与抗寒性的关系。两种处理均诱导兴津柑桔苗抗寒力的发育。在抗寒力发育过程中,叶片内源ABA、ABA/GAs在第7天呈一峰状剧增趋势。同时,其基因表达发生改变,第7到21天,新产生了32、25KD两种可溶性特异蛋白质及104、37、30KD3种膜特异蛋白,第14和21天产生17和82、27KD3种膜特异蛋白,它们在17～28℃脱锻炼3天后均消失。作者提出低温、ABA首先诱导ABA/GAs的积累,由ABA/GAs启动特异性板机,最终合成抗寒特异蛋白质,进而提高柑桔之抗寒力;膜特异蛋白质贡献可能更大。

Cold resistant Satsuma mandarin (Citrus unshiu Marc.cv. kotsu) was used to study the regulation of endogenous ABA / GAs on specific proteins by cold acclimation or ABA treatment, and the relationship between specific proteins and its cold resistance. Both the treatments induced 'Kotsu'cold resistance development. Endogenous ABA and ABA / GAs increased rapidly and reached peaks on the 7th day during acclimation or ABA treatment, and then dropped dramatically. Meanwhile, gene expression changed. Two kinds of soluble specific proteins (M. W. 32, 25KD) and three of membrane specific proteins (M. W. 104, 37, 30KD) were synthesized on the 7 ~ 21th day, and one of membrane specific proteins (M. W. 17KD) appeared on the 14~21th day, others (M. W. 82, 27KD) on the 21th day. They all disappeared after 3 days de-acclimation ( 17~ 28℃ ) . It is suggested that low-temperature and exogenous ABA brought about elevated levels of ABS / GAs, which in turn triggered the synthesis of specific proteins responsible for cold resistance, among which membrancc specific proteins might play a more important role.