光对柑桔组培无性系芽梢的生根效应

The Effect of Light on the Rooting of Clonal Shoots of Orange (Citrus Reticulate) in Vitro

以建水无籽蜜桔(Citrus reticulate)4个幼胚繁殖的芽梢为材料.实验结果表明: (1)芽梢接种后经4天暗培养再置于白光下继续培养的比未经暗处理的能提高生根率3.8％,提高生根势63.9％.若延长培养时间,则有明显的抑制作用. (2)3000Lux的白色光强有益于生根. (3)光谱中650nm的单色红光(光强300～430Lux)比白色光能提高生根率25.3％～39.7％,提高生根势47.3％～51.4％,且能加速生根进程和根系生长.红光主要作用时间在接种后的第4天以后.550 nm的单色黄光(光强443～1800 Lux)与白光相比,效果不太明显.410 nm的单色蓝光在低光强(460 Lux)时具有一定正效应.当光强增至600 Lux时,往往具有负效应.

The sterile multiplied shoots of juvenile embryo of jian Shui seedless orange (Citrus Reticulate) were used as experimetnal materials. The results showed: (1) The sterile shoots inoculated to rooting culture medium, after 4 days in the dark and then in the white light, the rooting rate of shoots were 3.8% and rooting potential were 63.9% higher than those only in the white light, the rooting potential were significantly increased. The rooting was obviously inhibited if delayed dark treatment time. (2) The rooting was satisfied at white light intensity 3000 Lux. (3) The 650nm monochrome red light (light intensity 300～430 Lux) played an important role in raising rooting rate and rooting protential as well accelerating rooting process and root growth. In monochrome red light, the rooting rate and rooting potential were 25.3%~ 39.7% and 47.3%～51.4% respectively higher than those in white light. Its main effects were on the four days later after inoculation. No significant change was obseved at 550nm (light intensity 443～1800 Lux) monochrome yellow light contrasted with those at white light. The 410 nm monochrome blue light presented a positive effect at tow light intensity (460 Lux) and had a negative effect when the light intensity increased above 600 Lux.