甘蓝型油菜茎尖培养中培养基成分对玻璃化作用的影响及玻璃化苗回复成正常苗的研究

INFLUENCE OF MEDIUM COMPONENTS ON VITRIFICATITION AND STUDIIES ON RECOVERY OF VITRIFIED SHOOTS TO NORMAL SHOOTS IN OIL RAPE SHOOT TIP CULTURE

甘蓝型油菜茎尖培养在琼脂浓度不同、大量元素和微量元素不同或附加马铃薯汁、活性碳和水解酪蛋白的培养基上形成大量的玻璃化苗,玻璃化苗大部分能回复成正常苗。每组实验的回复曲线基本“平行”,但各组实验间的回复曲线却截然不同。提高MS培养基中锰离子、钙离子含量时,玻璃化苗比例降低;而提高硼含量时玻璃化苗比例上升,微量元素对玻璃化作用的影响并非是通过改变培养基的水势而起作用的,提高维生素含量时,在MS培养基上能降低玻璃化苗比例;而在B_5培养基上,随着维生素含量的升高,玻璃化苗比例先是升高后是降低。

A large number of vitrified shoots were formed on media with different concentration of agrose, macroelement and microelement respectively;or on media supplemented with potato iuice, activated charcoal and casein hydrolysate, respectively, from the shoot tip culture Brassica napus. Most vitrfied shoots can reverse to normal shoots. The reversing cures of each set of experiments"paralled" cach other whereas the reverssing curves of various sets of experiments were much different.The ratio of vitrified shoots reduced when the level of Mn^(2+), Ca^(2+) of MS medium were raised, while the ratio of vitrified shoots increased by raising B level. The ratio of vitrified shoots can be reduced on MS medium by increasing vitamin level, first increased and then decreased on B_5 medium with raising vitamin level.