131Ⅰ－McAbJH_（107）在载人肝癌BEL－7402裸鼠体内的放射免疫显像分析

Analysis of Radioimmunological Image of131I-McAbJH107 in Nude Mice BearingHuman Liver Cancer BEL-7402

用氯胺Ｔ碘标技术标记抗人肝癌单克隆抗体ＪＨ１０７（ＭｃＡｂＪＨ１０７），观测其在载人肝癌ＢＥＬ７４０２裸鼠模型上的分布显像情况，氯胺Ｔ碘标法标记率５０％。每鼠腹腔注射２００Ｃｉ１３１Ⅰ－ＭｃＡｂＪＨ１０７，放射性物质第２４ｈ开始在肿瘤部位浓聚、逐步加强，９６ｈ达高峰，肿瘤组织较清楚显像并维持至１４８ｈ；同时周围组织放射性本底逐步减弱、消失。对照组１３１Ⅰ－ＮＩｇＧ呈全身均匀分布。无明显放射性物质浓聚及清楚显像。在４８ｈ和９６ｈ，１３１Ⅰ－ＭｃＡｂＪＨ１０７在１２种正常组织（脑除外）的Ｔ／ＮＴ均值分别为３．３８和６．２６，而１３１Ⅰ－ＮＩｇＧ的Ｔ／ＮＴ均值均低于１．０。

he monoclonal antibody JH107,(McAbJH107)against human liver cancerwas labelled by the ch-T131 I-radiolabelling method. Its distribution andimage were detected in the nude mice bearing human liver cancer BEL-7402. Injecting 200 1 131 I-McAbJH107 to each mouse peritoneally the con-centrating of the radioactive substance at the site of the tumor began at24th and strengthened progressively, and reached its top at 96th. Theclearer image of tumor tissue was kept to 148th. At the same time,theradioactive background of the tissues around the tumor gradually disappea-red. In131I-NIgG of the control group well-distributed in the whole body,no significant concentration and no clear image were observed. At 48 and96th, T/NT mean values of 131I-McAbJH107 in normal tissues of 12 kinds(except the brain ) were 3.38 and 6.26 respectively, and ones of 131I-NIgGwere lower than 1.0