犬弥漫性肺间质性病变动物模型的建立

The Reproduction of Animal Model withPulmonary interstitial Disease in Dogs

为了满足肺间质性病变影像学基础研究的需要，我们用（１）Ｃｏ￣（６０）照射法，（２）静脉内注射乎阳霉索法，（３）Ｃｏ（６０）照射和静脉内注射平阳霉素法，在２１只犬中复制了肺间质性病变的动物模型，６０ｄ后处死全部动物。病理学表现：（１）肺泡管、肺泡囊和肺泡间隔充血、水肿、细胞浸润和肺泡内的浆液性渗出。（２）肺泡内浆细胞堆积，肺泡皆破坏和塌陷，以及纤维组织增生。（３）支气管血管壁水肿、疏松、浆液性渗出，炎细胞浸润和纤维组织增生。血管间质旁组织也有充血、水肿、炎细胞浸润和纤维组织增生，（４）中央支气管和小动脉充血、水肿、鞘膜下结缔组织增生。肺内羟脯氨酸含量增加（Ｐ＜０．０００１），在三种方法之中，第三种方法最好。这种动物模型可应用于ＣＴ、ＨＲＣＴ、ＤＳＡ和ＭＲＩ等影像学及其病理基础的研究。解放军三十五医院放射科２组织病理学检查为了获得病理学标本，在实验３０ｄ时各组均处死一只犬。实验６０ｄ处死全部动物。用改良Ｈｅｉｔ１ｍａｎ氏膨胀固定法作充气固定肺标本，采用石蜡包埋。组织大切片制成２０～３０ｍ。小切片制成４～６ｍ。经ＨＥ染色和ＶａｎＧｉｅｓｏｎ氏胶原染色。３肺组织羟脯氨酸含量测定实验６０ｄ处死犬后取肺组织前、中、背部?

n order to meet the relevant basical research on image of pulmonaryinterstitial disease, 21 dog mode is of pulmonary interstitial diseasewere reproduced by using l.Co60 radiation, 2. intravenous of Bleomycin A5hydrochoridum, 3.by using both above. On 60 days later,all the dogs werekilled. Pathologic findings were as follows: l. Congestion and edema ofalveolar dilct, cyst and septum, celluar infiltration, and serous exudationof alveoli. 2.Accumulation of intra-alveolar destruction. collapse and fib-tous hyperplasia of alveoli. 3. Edema, rarefaction, serous exudation.cellular infiltration, fibrons hyperplasia of bronchovascular wall, andcongestion and edema of peri-interstium. 4. Congestion and edema of cent-ral btonchiolus and artery, rarefaction, edema and fibrosis of connectivetissue of sheath. The content of lung hydroxyproline increased(P<0.000l),three methods for hydraxyproline, the third one is the best.These animalmodels can be used on image research of CT, HRCT, MRI and DSA.