肿瘤颅内外转移机理的实验性探讨

A Study on Tumor Metastasis Outside andInside the Cranium

众所周知，胶质瘤很少发生颅外转移，但许多肿瘤却容易转移入颅内，本文以动物实验方法探讨这种现象发生机理。ＢＡＬＢ／Ｃ裸小鼠给予左侧颈内动脉注射人脑胶质瘤及黑色素瘤株细胞（１－２×１０／ｍｌ细胞悬液）后饲养观察。半月处死全部裸小鼠并对其进行大体及组织学检查。黑色素瘤颅内转移瘤成瘤率达１００％。而有部分胶质瘤却只能在颅外成瘤后少数破坏颅骨才能进入颅内，对照组采用胶质瘤作腹腔注射，结果８例中有５例发生肺部转移。本实验说明黑色素瘤只有很强的通过血脑屏障能力而胶质瘤细胞本身具有穿透血脑屏障以外的其它血管壁的浸润转移能力，而原发于颅内的胶质瘤不发生颅外转移很可能与其不能通过血脑屏障有关。

uman glioma rarely metastasizes outside the cranium but sometumors are highly metastat ic to thebrain. We developed an in-vivo modelinnude mice to study the metastatic mechanism to prevent glioma but allowmelanoma to pass through the blood-brain barrier. The glioma cellssuspension and melanoma cells suspension were injected in internal carotidartery of nude mice respectively.The glioma group resulted in metastatictumors invading the skull without passing the blood-brain barrier into thebrain. In the melanoma group tumor metastasized into the brain throughblood-bra in berrier in all cases.The tumor metastasis outside or inside thebrain is likely due to its ability to emigrate through the blood-brainbarrier. Direct carotid artery injection in nude mice is technically quitedifficuIt, but the animal model is useful to define cellular genetic, andimmunogical factors involving metastatic brain.