庆大霉素新剂型—水杨酸庆大霉素的耳毒性研究

A Study for Ototoxicity of A New Chemical Preparation - Salicylate Gentamicin

目的:探讨庆大霉素新剂型—水杨酸庆大霉素的耳毒性作用。方法:将实验动物随机分为五组,即庆大霉素(GM)组;水杨酸庆大霉素(SAGM)组;庆大霉素+水杨酸钠(GM+NaSA)组;水杨酸钠(NaSA)组和对照(C)组。实验前后检测动物ABR反应阈;耳蜗铺片观察毛细胞缺失程度;电镜观察毛细胞超微结构;并检测血清和外淋巴铁及肾功能。结果:SAGM组ABR阈移较GM组小(P<0.05),各频率和时间点与(GM+NaSA组无明显差异(P>0.05)。耳蜗铺片和电镜观察显示,SAGM组毛细胞损失程度和超徽结构变化比较GM组明显减轻。各组间血清铁和外淋巴铁无显著差异,SAGM对肾功能无明量影响。结论:新剂型水杨酸庆大霉素的耳毒性较硫酸庆大霉素明显减轻。

Objective: To investigate the ototoxicity of a new chemical preparation - salicylate gentamicin. Methods: Animals were arranged into the different groups, gentamicin (GM); salicylate gentamicin (SAGM); gentamicin+sodium salicylate (GM + NASA); sodium salicylate (NASA) and saline (C) were used respectively; ABR threshold, cochlea preparation, electronic microscopy and serum ferrum were tested to evaluate the ototoxicity of this preparation. Results: ABR threshold in SAGM was less than in GM (P<0.05). The loss of hair cells and ultrastructure were not so notable in SAGM then in GM (P>0.05). Ferrum in serum and perilymph were not significant between groups. Conclusion: The ototoxicity of the new chemical preparation is less than that of gentamicin.