供血者性别对血小板激活标志物含量的影响

Effect of donor gender on the activation marker levels of platelet

目的 探讨浓缩血小板保存过程中血小板激活的性别差异。方法 采用PRP法制备浓缩血小板,并于常规条件下保存5天,间隔取样,分别用RIA、ELISA方法检测血小板表面P-selectin分子数及血浆中可溶性P-selectin(sP-se-lectin)含量。结果 血浆中sP-selectin含量在保存1天后即明显升高,而血小板表面P-selectin分子数在保存3天后明显升高,二者与保存前比较均有显著性差异(P<0.05)。在保存期内,男女供血者的血小板表面P-selectin分子数之间比较无统计学差异(P>0.05),而女性供血者血浆中sP-selectin含量明显高于男性(P<0.05)。结论 在相同刺激因素影响下,女性供血者之血小板更易释放sP-selectin,因此在应用sP-selectin作为浓缩血小板质控指标及临床疾病的诊断时,应考虑供血者性别差异。

Objective To explore the difference of gender on activating platelet during storage. Methods PRP was used to produce concentrated platelets and we preserved them for five days in the condition of routine,then the number of P-selectin and content of soluble P-selectin were tested by RIA,ELTSA with septal sampling. Results The content of sP-selectin increased significantly after one day,and the number of P-selectin also increased significantly after three days(P <0. 05). There wasn't difference on P-selectin within blood donors during storage(P>0. 05) ,but the content of sP-sclectin was higher significantly on female. Conclusion It was easier for the platelet of female donor to release sP-selectin. And when we used sP-selectin as the quality control index of concentrated platelet and diagnosis of clinical disease.the effeet of gender should be considered.