不同方法制备浓缩血小板保存过程中细胞因子含量的检测

Changing of cellular factor in concentrated platelet by different prepared methods

目的 研究不同方法制备的浓缩血小板在保存过程中细胞因子含量的变化。方法 应用PRP法、BC法及SD法制备浓缩血小板,并于常规条件下保存,间隔取样检测保存期内细胞因子如IL-1β、IL-6、IL-8、TNF-α等的含量。结果浓缩血小板在保存期内随保存时间的延长,细胞因子含量升高,且浓缩血小板中细胞因子含量与其中混杂的白细胞数直接相关。结论 细胞因子在非溶血性发热输血反应发生中可能起协同作用。

Objective To study the changing of celluar factor in concentrated platelet by different prepared methods. Methods PRP,BC and SD were used to prepare concentrated platelets which were preserved in ordinary condition. And celluar factors (IL-I,IL-6,TL-8,TNF-a)were detected by intermittent sampling. Results The content of cellular factors improved with the increasing time of preservation.and there is direct relation between the cellular factors and mixed leukocyte. Conclusion Celluar factor may be cofactor on NHFTR.