应用NBT、TTC检测尿标本细菌总数方法的初步探讨

PRELIMINARY STUDIES ON THE TEST METHODS OF BACTERIA COUNT IN URIC SPECIMEN WITH NITROBLUE TETRAZOLIUM MEDIUM AND TRIPHENYLTETRAZOLIUM CHLORIDE MEDIUM

近年来尿标本细菌定量培养引起普遍的重视,有人将倾注培养法推荐为标准法。但是计数时在培养基不同层次中的小菌落识别困难,容易遗漏,加大计数误差。本文根据绝大多数细菌具有氧化酶,在培养基上能产生脱氢作用,使氯化三苯四氮唑(TTC)呈现红色或氯化硝基四氮唑蓝(NBT)呈现蓝色,试用 TTC 和 NBT 营养琼脂培养法做细菌计数培养,小菌落也能正确识别,不致漏检,从而提高计数的准确性。本文采用 TTC 营养琼脂、NBT 营养琼脂与营养琼脂法作对比观察其结果显示:TTC 的合适浓度为0.0025mg/ml、NBT 的最佳浓度为0.006mg/ml,并对其它有关影响计数准确性的因素进行了初步探讨,认为TTC 或 NBT 营养琼脂法较营养琼脂倾注培养法好。而 TTC 营养琼脂法优于 NBT 营养琼脂法。

In recent years,people have universally altached great importance to bacterial quantitative culture of uric specimen.Someone reco— mmended the pour into culture method,and thought it was a standard method.But the micro—colonies were difficultly distinguished and easily omitted.So that the count error increased。 This paper based on the theory that a great many bacteria had oxidase, and could produce dehydrogenation,and as a result that tripenyltetrazolium chloride(TTC)appeared as red and nitroblue tetrazolium(NBT)appeared as blue,so TTC and NBT nitrient agar media were used in bacteria count of uric specimen.Some micro—colonies could be correctly distinguished and fully counted Thereby,accuracy was raised.Our test result showed the TTC and NBT nutrient agar methods were better than nutrient agar pour into culture method.The best method was TTC untrient agar methed.The optimum dosages of TTC and NBT were 0.0025mg/ml and 0.006mg/ml in media,respectively。