抗氧化剂治疗大鼠肾病模型的初步探讨

The effect of antioxidant on adriamycin indueced nephropathy in rats

目的：探讨抗氧化剂对大鼠肾模型治疗作用。方法：给大鼠１次性静脉注射阿霉素（ＡＤＲ）制作肾病模型。选用抗氧化剂维生素Ｅ（ＶＥ）为治疗因素，测定血清中脂质过氧化物（ＬＰＯ），红细胞超氧化物歧化酶（ＳＯＤ）水平及相关血尿生化指标。结果：模型物组动物实验第１４天出现典型肾病综合征表现，血清中ＬＰＯ水平明显升高、红细胞ＳＯＤ活性明显降低。ＶＥ治疗组动尿、血生化指标及病理改变明显改善，且血清ＬＰＯ水平明显降低，红细胞ＳＯＤ活性明显高于ＡＤＲ组动物。结论：氧自由基，脂质过氧化损伤与ＡＤＲ肾病发生密切相关，ＶＥ能通过清除自由基。

Objective To explore the effect of antioxidant on nephropathy in rats.Methods A single intravenous injectin of abriamycin(ADR)results in marked proteinuria and morphological changes that aresimilar to minimal change nephrosis in humans.The effect of Vitamin E(VE) on ADR-induced renal injury in rats was examined.The serumlipidperoxide(LPO)level and the superoxide dismutase(SOD)activity of the erythrocyte was examined.Results The full expression of the syndrome occured at the 14th day after using ADR.The serum LPO level wassignificantly higher than that of the nomal cormal control group(P<0.01).Concurrent administration of VE showed amelioration of the normalcontrol group(P<0.01).Concurrent administration of VE showeda melioration of proteinuria and serum biochemical indexes.There were also less severe glmerular morpholgical changes in the VE group versus ADR group.The serum LPO level was singnificantly lower,the SOD activity of erythrocyte was higher than those in the ADR group(P<0.01).Conclusion This study suggests that the protective effect of VE may contribute to its ability to seavenge free radicals and inhibit lipidperoxidation.