摘要
采用TC199和RPMI1640培养基,加入FudR和咖啡因诱导剂,分别对14例fra(X)阳性个体和6例Fra(X)阴性个体进行脆性X染色体表达率对比研究发现:两种方法均能检出Fra(X)染色体,其表达率无明显差异(0.2<P<0.5);而用RPMI1640培养制作的标本明显优于TC199培养制作的标本,前者计数100个中期分裂相所需玻片数为3.4张,而后者需11.1张,两者比较有显著意义(P<0.001)。
By using two different culture media (TC 199 and RPMI 1640),the authors examined 14 persons with fragile X positive and six fragile X negative persons.FudR and caffeine as inducing agents were included in both. Fragile X couled be detected in either of them. The expression rate of fragile X with the two methods had no significant difference(0.2<P<0.5),but the RPMI 1640 medium was superior in specimen quality. In average,only 3.4 slides were required for analyzing 100 division cells by using RPMI 1640 medium as compared to 11.4 slides in the other The difference was highly significant(P<0.001).The result shows that RPMI 1640 medium may replace TC199 for testing fragile X chromosome.
出处
《中国优生与遗传杂志》
1994年第F08期29-31,共3页
Chinese Journal of Birth Health & Heredity