不同培养基脆性X表达率研究

Srudy of fragile X chromosome expression with different culture media

采用ＴＣ１９９和ＲＰＭＩ１６４０培养基，加入ＦｕｄＲ和咖啡因诱导剂，分别对１４例ｆｒａ（Ｘ）阳性个体和６例Ｆｒａ（Ｘ）阴性个体进行脆性Ｘ染色体表达率对比研究发现：两种方法均能检出Ｆｒａ（Ｘ）染色体，其表达率无明显差异（０．２＜Ｐ＜０．５）；而用ＲＰＭＩ１６４０培养制作的标本明显优于ＴＣ１９９培养制作的标本，前者计数１００个中期分裂相所需玻片数为３．４张，而后者需１１．１张，两者比较有显著意义（Ｐ＜０．００１）。

By using two different culture media (TC 199 and RPMI 1640),the authors examined 14 persons with fragile X positive and six fragile X negative persons.FudR and caffeine as inducing agents were included in both. Fragile X couled be detected in either of them. The expression rate of fragile X with the two methods had no significant difference(0.2<P<0.5),but the RPMI 1640 medium was superior in specimen quality. In average,only 3.4 slides were required for analyzing 100 division cells by using RPMI 1640 medium as compared to 11.4 slides in the other The difference was highly significant(P<0.001).The result shows that RPMI 1640 medium may replace TC199 for testing fragile X chromosome.