基因探针监测巨细胞病毒潜伏感染的母儿间传播

Surveyed CMV lacent infection in pregnancies and their infants by hybridization

本文采用基因探针检测孕妇静脉血及新生儿脐静脉血白细胞中ＣＭＶ－ＤＮＡ，同时应用ＥＬＩＳＡ检测其血清中ＩｇＧ、ＩｇＭ，结果：１０例孕妇ＣＭＶ－ＤＮＡ阳性，占８．７％（１０／１１５），这１０例孕妇血ＩｇＧ均阳性，ＩｇＭ均阴性；１７例新生儿ＣＭＶ－ＤＮＡ阳性，占１４．３％（１７／１１９），其中ＩｇＭ阳性２例，占１．７％（２／１１９）。本次研究中配对者７９例，母儿ＣＭＶ－ＤＮＡ均阳性者８例，新生儿ＣＭＶ－ＤＮＡ阳性而母亲阴性者４例。上述ＣＭＶ－ＤＮＡ阳性者均无临床症状。研究结果提示基因探针可监测ＣＭＶ的潜伏感染，从而进一步研究ＣＭＶ感染的母儿传播机制。

Cytomegalovirus(CMV) DNA were detected in white blood cells from pregnancies and their infants by hybridization. Result 10 of the 115 pregnancies were positive(8.7%).17 of the 119 infants were positive(14.3%).In this study, matched 79cases, there were 8 cases positive both pregnancies and their infants.4 cases infants positive,while their mothers were negtive.All the objects in the study were no significant clinical manifestation.This report showed hybridization may survey CMV lacent infection in pregnancies and their infants.