早期妊娠胚胎先天性人巨细胞病毒感染检测及危险因素分析

Test of congenital HCMV in fection in embryonic villi and analysis of dangerous factors

本文利用免疫组织化学方法（ＰＡＰ）和ＥＬＩＳＡ法对４００名早期妊娠胚胎绒毛组织进行人巨细胞病毒（ＨＣＭＶ）先天性感染和孕妇活动性ＨＣＭＶ抗体检测。结果表明早孕绒毛中有６７例感染，阳性率为１６．８％；孕妇血中ＨＣＭＶ－ＩｇＭ（＋）２２例，ＨＣＭＶ－ＩｇＡ（＋）２７例，孕妇活动性ＨＣＭＶ感染率为１０．０％。并对早期妊娠胚胎感染ＨＣＭＶ危险因素进行分析，孕妇活动性ＨＣＭＶ感染传播给胚胎的危险度为３２．５％，而且胚胎先天性感染与妊娠妇女的妊娠天数和性生活频度有着密切的相关关系。证明孕早期胚胎ＨＣＭＶ感染既可由母亲活动性ＨＣＭＶ感染垂直传播，也可来源父亲的传染途径。

Using peroxidase antiperoxidase and ELISA,congenital human cytomegalovirus(HCMV)in embryonic villi and the specific antibodies(IgM and IgA)in early pregnant women's serum were tested in 400 cases.Our results showed that the positive rate of HCMV antigen n early pregnant embryos was 16.8% and the rate of active HCMV infection in early pregnant women was 10.0%. The dangerous factors of HCMV infection in early pregnant women's embryos were analysed. The percentage of HCMV infection transmitted from prenant women with active HCMV infection to their embryos was 32.5%.The congenital HCMV infection rate of embryos was closely related to the pregnant stages and the frequency of sexual life. It indicated that congenital HCMV infection of embryos was not only from mothers'active HCMV infection, but also from fathers'indirect infection.