TORCH与优生：V．PCR方法检测人类细小病毒B19

TORCH and birth health: Detecrtion of human parvovirus B19 by PCR Method

套式ＰＣＲ方法可以用来检测人类细小病毒Ｂ１９（Ｂ１９）ＤＮＡ。利用特异性引物扩增得到Ｂ１９片段，对第一轮扩增产物进行ＤＮＡ印迹及限制性内切酶图谱分析，证明该产物是Ｂ１９ＤＮＡ的特异顺序。本文同时还利用地高辛标记寡核苷酸探针与ＰＣＲ产物杂交，可以检测少至１ｆｇ的Ｂ１９ＤＮＡ。用此套式ＰＣＲ方法对７例临床畸胎的组织标本检测，发现２例为Ｂ１９ＤＮＡ阳性。作者以为，此方法适用于快速、灵敏、特异地临床检测Ｂ１９ＤＮＡ。

The nested PCR was described for detecting human parvovirus B19(B19)DNA.Two pairs of specific oligonucleotides were designed.synthsized and used as primers to amplify B19 DNA.A specific amplified band with expect size(699 bp)of B19 DNA was shown on the agarose gel after the PCR product subjecting to gel electrophorosis.This amplified band was further proved to be specific B19 DNA sequence by Sourthern blot and restriction map analysis.The sensitivity to detecting quantity of B19 DNA is about 1fg.To assay clinical speciments,2 of 7 cases of abnormal dead fetal tissues were PCR positive.No doubtly,this is a sensitive,rapid method for detecting human parvovirus B19 DNA.