人子宫内膜细胞体外培养及其生物学特性检测

IN-VITRO CULTURE OF HUMAN ENDOMETRIAL CELLS AND EXAMINATION OF ITS BIOLOGICAL CHARACTERISTICS

用0.2％胰蛋白酶消化分离人子宫内膜腺体和单个细胞,行体外原代和传代培养均获得成功。台盼兰染色活细胞达90％以上,细胞种植12～16h附壁生长,5～7天长成单层,传代间隔4～5天。该细胞具有雌、孕激素受体,雌二醇或与孕酮联用能刺激细胞生长,孕酮则抑制其生长,且保持分泌前列腺素的功能。结果表明,人子宫内膜细胞体外培养是一种简单稳定的方法。该细胞可广泛地应用于妇产科、计划生育等领域的研究。

The isolation and in vitro culture of human uterine endemotrial gland and cells was attempted. Human uterine endometrium was dissociated by 0.2％ trypsin for primary culture and subculture with a viability rate of the cells up to 90％ as judged by the Trypan blue exclusion test. Endometrial cells showed anchoraged growth after 12～16 hours, and grew into monolayer after 7 days. A series of sub- culture had been performed for about 4～5 days. Steroid receptors and prostaglandin production could be identified. Cell growth was promoted by estradiol and inhibited by progesterone. Combined treatment of estradiol and progesterone promoted cell growth as well. These results show that the in-vitro culture of human endometrium is a stable and useful method.