江苏大麦纹枯病菌酯酶同工酶的测定

DETERMINATION OF ESTERASE ISOENZYMES OF RHIZOCTONIA CEREALIS AND RHIZOCTONIA SOLANI FROM BARLEY IN JIANGSU

对立枯丝核菌(Rhizoctonia solani Kühn)和禾谷丝核菌(R.cerealis Vander Hoeven)的16个菌丝融合群或亚群的标准菌株及来自江苏大麦纹枯病的9个菌丝融合群或亚群共68个菌株进行了聚丙烯酰胺凝胶电泳,测定酯酶同工酶。结果表明:1.立枯丝核菌主酶带数的变幅范围比禾谷丝核菌大;2.无论禾谷丝核菌,还是立枯丝核菌,各菌丝融合群或亚群之间的电泳图谱都有显著差异,但与各自对应的融合群或亚群的标准菌株的图谱则相似;3.44个大麦禾谷丝核菌CAG-1群菌株间的图谱也有差异,但都有一条共同的主酶带(E_4);4.据主副酶带数目和位置,将禾谷丝核菌CAG-1群菌株再分为2个类型(Ⅰ型和Ⅱ型)和5个亚型(Ⅰa、Ⅰb、Ⅰc和Ⅱa、Ⅱb);酶谱类型与菌株的采集品种和致病性无关,但与采集地点似有一定的联系。

The esterase isoenzyme patterns of 16 standards strains in 16 AGs or sub-AGs and 52 solates from barley in 9 AGs or sub-AGs of Rhizoctonia cerealis and R.solani were ana- lysed with the slab polyacrylamide gel electrophoresis.The results showed:1.Main bands of R.solani were more variable than those of R.cerealis;2.there were marked differences among the patterns of 9 AGs or sub-AGs of R.cerealis and R.solani from barley but similar patterns were shown within AGs or sub-AGs;3.there were some differences among the esterase isoen- zyme patterns of 44 isolates in CAG-1 of R.cerealis from barley but a common main band (E_4) was shaown;4.according to the number and relative position of esterase isoenzyme bands 44 isolates in CAG-1 may be classified into 2 types and 5 sub-types.The esterase patterns had no relation to the pathogenicity or host varieties but they were related to the geographic origins.