摘要
从73个尖孢镰孢(Fusarium oxysporum)不同专化型菌株上获得684个硝酸盐营养突变株(nit mutant)。作相关氮源利用试验及亚硝酸反应后,鉴定出一新硝酸盐营养突变类型:亚硝酸盐还原酶结构基因类型,命名为nit8,占总突变株的6.7%。同时被鉴别的还有nit1、nit3和Nit M三种突变类型,它们分别占突变株总数的81.0%,3.8%和8.5%。此外,首次引入一种亚硝酸反应在这类研究中的应用,还提出了互补指数概念与公式来表示nit突变株营养体之间亲和的能力。
A new nitrate nonutilizing (nit)mutant phenotype,named nit 8, reflecting mutation presumptively in a structural gene for nitrate reductase was de- termined in Fusarium oxysporum isolates.In addition to nit 8,phenotypes nit 1, nit 3 and Nit M were also identified among 684 nit mutants discovered from 73 isolates of F.oxysporum and their isolation frequencies were 6.7,81.0,3.8 and 8.5%, respectively.A color indicating method in nitrite excretion tests was firstly intro- duced in such kind of studies and a new concept with a formula on complementation index (CI) was proposed to express the vegetative compatibility in pairing tests be- tween nit mutants.
出处
《真菌学报》
CAS
CSCD
北大核心
1993年第4期297-303,共7页
基金
国家自然科学基金
关键词
尖孢镰孢
硝酸盐
突变型
营养
Fusarium oxysporum
Nitrate nonutilizing mutant
Phenotype
Vegetative compatibility
Complementation index
