辣椒素处理坐骨神经对大鼠痛阈和电针镇痛效应的影响

Influence of Capsaicin Treating Sciatic Nerve on the Pain Threshold and the Effect of Acupuncture Analgesia of Rats

本实验用1.5%辣椒素(Cap)处理大鼠右侧坐骨神经,溶媒(Veh)处理左侧坐骨神经的动物模型,通过自身对照观察了 Cap 对痛阈及电针(EA)镇痛效应的影响,并用组织化学方法,观察了 Cap 对动物脊髓后角抗氟化钠酸性磷酸酶(FRAP)活性的影响。结果发现:经Cap 处理后,与对照侧相比,实验侧痛阈明显升高,同时后角内 FRAP消失;电针“环跳”后,与电针前相比对照侧的针刺镇痛效应显著升高,实验侧的针刺镇痛效应明显减弱。表明一级传入 C 纤维不仅参与痛信息的传入,而且还参与针刺镇痛信息的传入。

The model of local application of 1.5% Capsaicin(Cap)on the right sciatic nerve and control ofVehicle(Veh)on the left were used.The influence of Cap on pain threshold,electroacupuncture(EA)analgesia and Floride-resistant acid phosphatase(FRAP)activity in the dorsal horn of spinalcord were observed.The results showed,the pain threshold of Cap treatment side rose significantlycompared with the control side,FRAP in dorsal horn of spinal cord vanished,and the analgesic effectof EA at“Huantiao”of the Cap treatment side markedly decreased,compared with not only that EAat the control side but also that EA before Cap treatment.It is indicated that the C fibers of the pri-mary afferent participate in the input of pain and EA analgesic information.