猫丘脑后核群内脏伤害感受神经元细胞内电位记录及HRP细胞内标记

Recording Intracellular Potentials of Viscerono-ciceptive Neurons in Posterior Group of Thalamic Nuclei(PO)and Their Labeling with HRP Intracelluiarly in Cats

用40只猫,以强的方波脉冲刺激内脏大神经向中纤维,记录丘脑后核群(PO)中相关神经元细胞内电位。共记录了42个单位。其静息电位为53.74±11.68毫伏,动作电位为29.44±9.22毫伏。随刺激强度变化可出现 EPSP、单峰及多峰的锋电位。此内脏大神经诱发的动作电位可被静脉注射吗啡所抑制。在胞内电位记录的基础上,用HRP 胞内标记了2个相关神经元和2个非相关神经元。初步比较了其形态差异。还看到刺激杏仁核,于 PO 中产生 IPSP,从而抑制了神经元诱发的胞内电位。

Intracellular potentials(ICP)of the neurons in PO evoked by stimulating greater splanchnicnerve(GSN)were recorded in 42 cats.The resting potentials and action potentials induced by stimu-lating GSN were 53.74±11.68 mV and 29.44±9.22 mV respectively.Owing to intensity of thestimulation we can record EPSP and single spike or polyspikes on it.The action potentials derivedfrom stimulating GSN(related action potentials)were inhibited by injection of morphine intravenous-ly.On the basis of recording ICP,we also labeled 2 related neurons with stimulation of GSN and 2unrelated ones.Differences between two kinds of the neurons were compared preliminarily.The ICPof the related neuron with stimulating GSN were inhibited by stimulating Amygdala too.Factors influencing amplitute of action potential in intracelluar recording,criteria of determiningnociceptive neuron and inhibition derived from stimulating Amygdala were also discussed.