文心兰茎尖离体培养研究

In vitro culture of Oncidium shoot tiop

文心兰茎尖培养的研究,试验结果表明:文心兰茎尖在MS+BA2mg/L+NAA 培养基上,离体培养个月后产生原球茎。改良基本培养较适原球茎的增殖;0.5mg/L1KC用的激素配比,原球茎月增殖率可达;可以减少原球BA1.5mg/L+NAA0.2mg/L332.5%CW茎褐死现象和促进原球茎的增殖;壮苗阶段,以附加香蕉汁的效果较好。NAA0.5%+10%水苔是良好的移栽基质,成活率在。85.3%

The study results of Oncidium shoot tip culture is reported in this paper. Experiment show that: in medium consist of MA+BA2mg/L+NAA0.5mg/L, after one months culture the shoot tip can produce Protocorm-like Boby; The reformed KC basial medium is good for the proliferation of Protocorm-likd Body; When the hormone composition is BA1.5mg/L+NAA0.2mg/L, Protocorm-like Bodys monthy prolieration rare can reach as high as 332.5% Cw can reduce brown death phenomena and enhance the proliferation; In shoot robusting period; add NAA0.5mg/L+10%Banana juice to the basic medium can obrain a good effect; Water live mosses is a very good matrix for transplats, the survival rate can reach 85.3%