摘要
应用聚合酶链式反应技术(polymerase chain reaction),在体外从玉米(Zea mays L.)基因组中扩增10 kD玉米醇溶蛋白基因的编码区,得到了一特异的0.57 kb的扩增产物,将其克隆后对此片段进行限制性内切酶图谱分析和全序列测定。结果证明,克隆得到的是完整的玉米醇溶蛋白基因编码区,与国外报道相比,其核苷酸序列及推测的氨基酸序列的同源率分别为96%和90%。含硫氨基酸在该蛋白中高达25%。将该基因导入牧草及其它农作物,以期得到改良产品的工作正在进行。
The results of cloning and sequencing of the gene enooding 10 kD zein of maize (Zea mays L.) with polymerase chain reaction (PCR) technique are here with presented. The genomic DNA template was extracted from sterilized seedlings of maize. Primered with a pair of synthetic 5′ and 3′ PCR primers, a 0.57 kb DNA fragment was obtained after 30 PCR amplification cycles. The restriction map of the DNA fragment has been determined. The result indicated that the entire coding sequence of 10 kD zein gene has been cloned. The homologies of the DNA sequence and deduced amino acid sequences between our result and those published abroad are 96% and 900% respectively. As 10 kD zein is rich in sulphur, this gene might be used to improve the quality of crops, especially, the forage legumes by genetic engineering.
基金
863项目
