摘要
国外曾报道利用悬浮培养细胞分离、培养甜菜原生质体,结果分别得到多细胞团或愈伤组织。国内有关甜菜原生质体培养尚未见报道。本文报道对悬浮细胞来源的甜菜原生质体的培养结果。
conditions appropriate for isolation and culture of protoplasts from cell suspension cultures of sugar beet (Beta vulgaris L.) were investigated.Protoplasts with high yields and high quality were obtained by treating cells with a mixture of cellulase,macerozyme R-10 and driselase,or other enzyme combinations.Protoplasts were cultured in MS liquid medium or solid agar medium.Callus was obtained from the cultured protoplasts.
基金
中国农业科学院院长基金资助项目
关键词
甜菜
原生质体培养
愈伤组织再生
Sugar beet (Beta vulgaris)
Protoplast culture
Callus regeneration
