条叶龙胆根培养物中龙胆苦苷的产生和含量

Gentiopiroside Production from Roots Cultures of Gentiana manshurica

条对龙胆愈伤组织中龙胆苦苷含量较低,根的分化可以提高龙胆苦苷的含量。培养的离休根中龙胆苦苷的含量则更高。随着继代次数的增加,愈伤组织中龙胆苦苷含量下降快,而分化根的愈伤组织和离体培养根中龙胆苦苷含量下降较慢。在愈伤组织的液体培养中,1/2MS培养基附加低浓度的植物激素,龙胆苦苷含量有所提高。植物激素配比促进愈伤组织中分化根的形成,也促进龙胆苦苷的产生;但促进愈伤组织的生长时,不利于龙胆苦苷产生。在离体根培养中,添加 1mg/L的金雀花碱,促进龙胆苦苷的产生,含量可达1.48％,明显高于愈伤组织(0.52％)和分化根的愈伤组织(0.65％),也高于试管苗根的龙胆苦苷含量(1.01％)。

Gentiopicroside content in calli ofGentiana manshurica Kitag was low. Itwas higher in the calli with differenti-ated roots, and the highest in excisedroots. Gentiopicroside content in calli,excised roots and calli with differenti-ated roots was decreased after continu-ous subculturing, but it was decreasedmore rapidly in the calli than the lattrtwo (Fig. 1). In callus suspension cul-ture, addition of low concentrations ofphytohormones to 1/2 MS medium hadno influence on callus growth but stim-ulated gentiopicroside production (Table1). Phytohormones with the ratio fa-voring the formation of roots couldpromote gentiopicroside production.On the other hand, the ratio stimulat-ing callus growth reduced gentiopicro-side production (Table 2). Cytisine atconcentration of 1 mg/L could stimu-late gentiopicroside production in ex-cised roots (Table 3) up to 1. 48% ofdry weight, which was higher thanthat in callus (0. 52%), in differenti-ated root-callus (0. 65%), and intube plantlet root (1. 01%).