棕榈花序中抗真菌蛋白质的分离及特性

Isolation and Characterization of An Antifungal Protein from Inflorescence of Trachycarpus fortunei W

棕榈花序用盐溶液提取,硫酸铵沉淀,透析,DEAE—Cellulose DE—52离子交换,超滤,再经 Ultrostl AcA—44柱过滤,上 Bio—Gel P—6柱后,分离到一个相对分子量为25.6 kD的蛋白质(TP—1)。TP—1在浓度低于1.0mg/ml即可明显抑制绿色木霉菌丝的生长,对赤霉菌、棉花枯萎菌及稻瘟菌都有抑制作用。TP—1无几丁酶和β—1,3—葡聚糖酶活性.也无血球凝集活性。但经蒽酮法及PAS反应证明是一个糖蛋白。氨基酸成分分析表明,TP—1富含A_(sD)、Gly、Ala和Leu。

A protein named TP-1 from in-florescence of Trachycarpus fortunei W.was isolated through a procedure in-cluding saline solution extraction, salt-ing-out by ammonium sulfate, dialy-sis, ion exchange chromatography, ul-trafiltration, and gel filtration on Ul-trogel AcA-44 and Bio-Gel P-6. Theprotein's molecular weight was 25.6kD determined by SDS-PAGE. OnPDA media, it inhibited the growth ofmycelia of Trichoderma viride at concen-tration of 1. 0 mg/ml. TP--1 also hadinhibitive activity against mycelialgrowth of some crop fungal pathogens,but no activity of chitinase, β-1, 3-glucanase, or hemagglutination. ByPAS reaction and anthrone test, it wasindicated that TP--1 was a glycoprote-in. Analysis of the composition ofamino acids showed that TP- 1 was richin Asp, Gly, Ala, Val and Leu.