摘要
报道了新疆紫草(Arnebia euchroma(Royle)Johnst.)细胞在悬浮培养和发酵培养中的某些特性。细胞悬浮培养的接种量以2.50g·干重/L较为合适,其相应的月产率为22.0g·干重/L。细胞生长的时间进程:接种后0—3天细胞生长停滞,3—12天迅速生长,12天以后基本停止生长。pH值随培养的时间而变化,接种后第12天出现一次高峰。在悬浮培养的生产培养基中,紫草宁衍生物的合成在接种后0—25天内迅速增加,25天左右达到高峰。发酵培养的月产率达到9.47g·干重/L和9.34g·干重/L,其细胞的生长动态与悬浮培养的相似。10L发酵培养的总色素含量达到干重的14.26％,紫草宁衍生物的产率为1.93g/L。
This paper reports some characteristics of cell suspension and fermentation culture in Arnebia euchroma (Royle) Johnst. The yield of suspension cultule reached 22.0g dry wt/L Per month when inoculum quantity was 2.50 g dry wt/L. Time-course study showed that cell gro- with lagged in 0—3 days and enhanced greatly in 3—12 days, and almost ceased after 12 days of culture. pH value changed during the culture period and peaked on the 12th day after inoculation. When cells were cultured in liquid production medium, the contents of shi- konin derivatives increased quickly and reached to the maximum about the 25th day. The cell yield of 9.47 and 9.34 g dry wt/L per month was obtained in fermentation culture. Time- course of cell growth in fermentation culture was similar to that in suspension culture. The total content of shikonin derivatives in fermentation culture was 14.26% dry weight from 10 L bioreactor. The yield of shikonin derivatives was 1.93 g/L.
基金
国家"七五"攻关课题
关键词
新疆紫草
悬浮培养
发酵培养
紫草
Arnebia euchroma
Suspension culture
Fermentation culture
Cell growth
Shikonin derivatives
