摘要
应用荧光探剂 1,6-二苯基-1,3,5-己三烯(DPH)观察外源胆固醇对水稻极端线粒体膜流动性的影响。结果表明,无论外源胆固醇通过水培根系吸收或是直接添加给予水稻根端线粒体,都能使DPH与线粒体结合后的荧光强度减弱,使荧光偏振度和微粘度降低,增加水稻根端线粒体膜流动性。
The effect of exogenous cholesterol on the fluidity of mitochondrial membrane from rice root apex was investigated using the DPH fluorescece probe (1,6-diphenyl-1, 3, 5,-hexatriene). Results showed that exogenous cholesterol, either being absorbed by root system during water culture of rice or being added directly to the prepared mitochondrial of control rice root apex, decreased the fluorescence intesity of probe in the mitochondrial membrane and reduced the fluorescence polarization as well as micro-viscosity, but increased the fluidity of the mitochou- drial membrane in rice root apex.
基金
国家自然科学基金
关键词
胆固醇
线粒体
膜流动性
水稻
Cholesterol
Fluorescence probe
Fluidity of mitochondrial membrane
