籼型光敏雄性不育水稻杂种花粉植株游离小孢子培养成植株

PLANT DEVELOPMENT FROM ISOLATED MICROSPORES OF ORYZA SATIVA L.SSP.INDICA DERIVED FROM THE ANTHER CULTURE OF F_2 HYBRIDS OF PHOTOPERIOD-SENSITIVE MALE-STERILE RICE

禾本科植物游离小孢子的培养已在水稻、小麦、玉米、大麦等主要农作物上获得成功,且在大麦、玉米上成功地从未经预处理及预培养的游离小孢子培养获得了再生植株。籼稻花药培养能力远远低于粳稻,对其游离小孢子的离体培养研究甚少。本文简要报道这方面的研究结果。

Microspores from a highly anther culturable rice line(Oryza sativa L.spp.indica)deri- ved from the anther culture of F_2 hybrids of photoperiod-sensitive male-sterile rice,after 7 days of low temperature treatment and another 7 days of preculture within anthers,were isolated m mechanically.They were cultured in N_6 medium containing 3% manitol,6% sucrose,5g/L ino- sitol,100 mg/L serine,800 mg/L glutamine,1000 mg/L L-proline,10%(V/V)coconut milk and 2 mg/L,2,4-D,and 1 mg/L kinetin.After 5 days,microspores initiated first division and subsequently developed into multicellular pollens and calli.Green plant could be recovered when compact calli were transferred onto agar-solidified MS medium containing 3% sucrose, 0.5 mg/L kinetin,2 mg/L 6-BA and 1 mg/L IAA.