摘要
在过去的几年中水稻(Oryza sativa L.)的原生质体培养取得了较大进展。我们在此基础上对国内大面积推广的优良粳稻品种的原生质体进行了高频植株再生的实验,并对两种不同的培养方法——琼脂糖包埋法和看护培养法进行了初步的比较研究。
Calli were induced from anther protoplasts in suspension cultures,and from mature emb- ryo derived callus.After transferring the calli into plant differentiation medium,plantles were obtained.The protoplast plating efficiency and plantlet regeneration frequency were influenced not only by rice(Oryza sativa L.)genotypes but also by the age of suspension lines. The F_2 anther-derived calli gave a protoplast plating efficiency of 4.5%—9.6% and plantlet regeneration frequency of 20.0%—36.7% using agarose-imbedded method.In comparative stu- dy of the agarose-imbedded culture and nurse culture,the latter method seemed to be more ad- vantageous in time saving and high efficiency.
关键词
水稻
原生质体
植株再生
Oryza sativa L
Protoplast
Plant regeneration
